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URL: http://www.addgene.org/19314
Proper Citation: RRID:Addgene_19314
Insert Name: Ras-related GTP binding B
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18497260
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI Note from the depositor: This plasmid does not express well. This is because it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells.
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No rating or validation information has been found for Flag pLJM1 RagB 54L.
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Source: Addgene
