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URL: http://www.addgene.org/194281
Proper Citation: RRID:Addgene_194281
Insert Name: dCas9-KRAB-GFP
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:37534178
Vector Backbone Description: Backbone Size:7000; Vector Backbone:FUGW; Addgene 71237; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Use Esp3I (type IIS restriction enzyme) for scarless insert of desired sgRNA downstream of U6 promoter. 5’ ggacG(N)20 3’ 5’ aaac(N’)20c 3’ Where N = 20 nt sgRNA sequence for your target gene ggac and aaac are the overhanging ends to complement the Esp3I cut p031. The extra g in the plus strand (and the complement c in the minus strand) is to promote RNA pol III transcriptional initiation. Please visit https://doi.org/10.1101/2022.10.31.514562 for bioRxiv preprint.
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Source: Addgene
