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URL: http://www.addgene.org/195039
Proper Citation: RRID:Addgene_195039
Insert Name: KanR
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36503721
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference. For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation" The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
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Source: Addgene
