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URL: http://www.addgene.org/195608
Proper Citation: RRID:Addgene_195608
Insert Name: PI-SceI
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7890714
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D218A mutation in the first LAGLIDADG motif that inactivates the protein.
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No rating or validation information has been found for pET23PI-Sce ESARC D218A.
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Source: Addgene
