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URL: http://www.addgene.org/195615
Proper Citation: RRID:Addgene_195615
Insert Name: I-SceI
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18644379
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K223I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus.
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Source: Addgene
