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Plasmid Name
pET15b (I-WcaINhis) K125I
RRID:Addgene_195619 RRID Copied  
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RRID:Addgene_195619
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Plasmid Information

URL: http://www.addgene.org/195619

Proper Citation: RRID:Addgene_195619

Insert Name: I-WcaI

Organism: Other

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Expresses the I-WcaI homing endonuclease from Wickerhamomyces canadensis. The sequence is codon optimized for expression in E. coli. The expressed protein contains a K125I mutation that results in DNA nicking activity. It contains an amino-terminal histidine tag for purification.

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Ratings and Alerts

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Data and Source Information

Source: Addgene