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Plasmid Name
BM28
RRID:Addgene_201047 RRID Copied  
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RRID:Addgene_201047
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Plasmid Information

URL: http://www.addgene.org/201047

Proper Citation: RRID:Addgene_201047

Insert Name: the groESL region region from E. coli is the insert in the pBR325 plasmid pOF39

Bacterial Resistance: Ampicillin and Kanamycin

Defining Citation: PMID:36973666

Vector Backbone Description: Vector Backbone:the pOF39 plasmid backbone is pBR325; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin

Comments: Please see the BM28 genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102380) and the accompanying papers for more details. Additional publications: Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180. And for the pOF39 plasmid: Fayet O, Louarn JM, Georgopoulos C. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes. Mol Gen Genet. 1986 Mar;202(3):435-45. doi: 10.1007/BF00333274. PMID: 3012269.

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Data and Source Information

Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org