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URL: http://www.addgene.org/20184
Proper Citation: RRID:Addgene_20184
Insert Name: Notch 2 intracellular domain
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16365048
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2 (5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3'). Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
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Source: Addgene
