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Plasmid Name
pGCNK58
RRID:Addgene_202461 RRID Copied  
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RRID:Addgene_202461
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Plasmid Information

URL: http://www.addgene.org/202461

Proper Citation: RRID:Addgene_202461

Insert Name: Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Defining Citation: PMID:2145515

Vector Backbone Description: Vector Backbone:pet3b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Dynamics of GCN4 facilitate DNA interaction: a model-free analysis of an intrinsically disordered region. Gill ML, Byrd RA, Palmer AG III. Phys Chem Chem Phys. 2016 Feb 17;18(8):5839-49. doi: 10.1039/c5cp06197k. The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted a-helices: crystal structure of the protein-DNA complex. Ellenberger, T. E., Brandl, C. J., Struhl, K. & Harrison, S. C. (1992). Cell, 71, 1223-1237. PMID 1473154.

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Data and Source Information

Source: Addgene