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URL: http://www.addgene.org/206874
Proper Citation: RRID:Addgene_206874
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:39036966
Vector Backbone Description: Backbone Marker:Dominic Esposito (Addgene plasmid # 11518); Backbone Size:8695; Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Useful bacterial expression vector for purifying challenging proteins. SuperHis (14xHis) ensures very high affinity to Ni-IMAC column. mOCR tag is very negatively charged. This promotes proper folding, prevents aggregation and enables purification via ion-exchange. Refer to DelProposto et al 2008 (DOI: 10.1016/j.pep.2008.08.011) for information on mOCR tag fusions.
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Source: Addgene
