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URL: http://www.addgene.org/206936
Proper Citation: RRID:Addgene_206936
Insert Name: spCas9
Organism: S. Pyogenes
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Sunghee Chai, Markus Grompe; Backbone Size:2963; Vector Backbone:pAAV-MP-84; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The DNA sequence of spCas9 including SV40 NLS tag (no HA tag) is from the pX551 plasmid sequence (Feng Zhang lab). spCas9 is expressed from MP-84 promoter and the gRNA sequence from a shorter version of human U6 promoter (miniU6). The gRNA contains a locked tracrRNA (t-lock) secondary structure (Riesenberg et al., Nat. Commun., 2022, 13:489).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
