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URL: http://www.addgene.org/20705
Proper Citation: RRID:Addgene_20705
Insert Name: Itk
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10636929
Vector Backbone Description: Backbone Size:0; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Wild-type Itk. See author's map for more information. The native stop codon of Itk was deleted and replaced with a PCR-derived fragment encoding a short linker peptide and a carboxyl-terminal Myc epitope tag. These modifications extend Itk by 21 amino acids (AGAEFMEQKLISEEDLRKFDI). EF/Itk-myc expression vectors were generated by inserting the modified Itk cDNA into a derivative of pEF-BOS.
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Source: Addgene