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URL: http://www.addgene.org/212035
Proper Citation: RRID:Addgene_212035
Insert Name: mPer2-luciferase
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39370744
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6339; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Comments: The insert (mPer-luciferase) derived from the pGL3 basic E2 vector (Plasmid #48747, a gift from Joseph Takahashi). The pLV6 backbone derived from the pLV6-Bmal-luc vector (Plasmid #68833, a gift from Steven Brown). The pLV6-Bmal-luc vector contains one BamHI cutting site 3’ of the Bmal1-luciferase insert. Site-directed mutagenesis was used to create an additional BamHI cutting site 5’ of the insert. The mPer2-luc insert of the pGL3-Per2-luc vector is by default flanked by BamHI cutting sites. After successful digestions and extractions, the pLV6 backbone and the mPer2-luc fragment were ligated with T4 DNA ligase, hence obtaining the pLV6-mPer2-luc vector.
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Source: Addgene
