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URL: http://www.addgene.org/213025
Proper Citation: RRID:Addgene_213025
Insert Name: LINE-1 ORF2p (1-1275)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38096902
Vector Backbone Description: Backbone Marker:Kacper Rogala, modified by Martin Taylor; Backbone Size:3541; Vector Backbone:pDARMO-PolH2.1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Includes polyhedrin terminator and backbone is designed to be useful in MultiBac scenarios. Note that relative to some insect vectors, the Tn7 sites are reversed, so the insert may be flipped in the destination bacmid and PCR primers may need to be swapped accordingly. The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). For more on pDARMO vectors see Rogala, K. B. et al. Structural basis for the docking of mTORC1 on the lysosomal surface. Science 366, 468-475 (2019). https://doi.org/10.1126/science.aay0166
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No rating or validation information has been found for pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1.
No alerts have been found for pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1.
Source: Addgene
