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URL: http://www.addgene.org/213845
Proper Citation: RRID:Addgene_213845
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:38906116
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Comments: Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10). To insert barcoded genes: i) digest this plasmid with BbsI and MfeI ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites iii) ligate the two digestion products. Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
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Source: Addgene
