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URL: http://www.addgene.org/21824
Proper Citation: RRID:Addgene_21824
Insert Name: abu-1 promoter
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12186849
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Addgene sequencing results show several mismatches with author's sequence. abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.
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No rating or validation information has been found for SREC 1: abu-1::gfp.
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Source: Addgene
