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URL: http://www.addgene.org/220248
Proper Citation: RRID:Addgene_220248
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:38911550
Vector Backbone Description: Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172). Please download the file linked below to obtain a well annotated map of the plasmid.
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Source: Addgene
