Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/220280
Proper Citation: RRID:Addgene_220280
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:38911550
Vector Backbone Description: Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome. Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates. Please download the file linked below to obtain a well annotated map of the plasmid.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pEC2796 (pSpy0C4).
No alerts have been found for pEC2796 (pSpy0C4).
Source: Addgene
