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URL: http://www.addgene.org/22089
Proper Citation: RRID:Addgene_22089
Insert Name: FGF8
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8548816
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK (-); Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region. For antisense probe linearize with EcoRI and use T7 Polymerase.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org