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URL: http://www.addgene.org/221004
Proper Citation: RRID:Addgene_221004
Insert Name: CasRx 30nt processed direct repeat with SD1 mutation
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38917795
Vector Backbone Description: Vector Backbone:pXR003 (Addgene #109053); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: The gRNA backbone contains the SD1 mutation (DR1 A>T) in the direct repeat, which boosted knockdown efficiency in a previous paper (Wessels et al., 2020, Nat Biotech). We have found increased splicing modulation with this mutation compared to the WT direct repeat.
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Source: Addgene
