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URL: http://www.addgene.org/221127
Proper Citation: RRID:Addgene_221127
Insert Name: pHluorin
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38809589
Vector Backbone Description: Vector Backbone:pFA6-HPHMX4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This pHluorin integration plasmid was constructed by subcloning the 977 bp SalI/BglII fragment from pBW1571 (pFA6a-pHluorin::KANMX6 from (Prosser et al., 2010) into the SalI/BglII sites of pAG32 (pFA6-HPHMX4 described in (Goldstein and McCusker, 1999). The resulting plasmid was used for C-terminal tagging of the endogenous STE3 locus using a PCR-based integration strategy described in (Longtine et al., 1998)
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Source: Addgene
