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URL: http://www.addgene.org/225496
Proper Citation: RRID:Addgene_225496
Insert Name: Lifeact
Bacterial Resistance: Kanamycin
Defining Citation: PMID:41495248
Vector Backbone Description: Vector Backbone:pENTR2b; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: First, gene blocks containing the lifeact sequence (Riedl et al., 2008) in frame with mNeonGreen (Shaner et al., 2013) and flanked with Kpn1 and Xho1 restriction sites were purchased from IDT (Integrated DNA Technologies, Inc.). Through enzymatic digestion (Kpn1, ThermoFisher Scientific, FD0524, and XhoI, ThermoFisher Scientific, FD0694) of the two sites on both gene blocks and the pENTR2b entry vector (ThermoFisher Scientific, A10463), we obtained pENTR2b-Lifeact-mNeonGreen. Please visit https://doi.org/10.1101/2024.09.30.615654 for the bioRxiv preprint.
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Source: Addgene
