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URL: http://www.addgene.org/226796
Proper Citation: RRID:Addgene_226796
Insert Name: nanos2
Organism: Danio rerio
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Nathan Lawson; Backbone Size:2810; Vector Backbone:p5E-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by Maya Pahima. The putative zebrafish nos2 promoter was cloned from zebrafish ovary gDNA from a region directly upstream of the nos2 coding sequence with the following primers: 3’-ACTGGCCTAAAGGGTGTCCT-5’ and 3’-CGTCCCTTGCCTTTAGTCTG-5’. The cloned amplicon was inserted into the pCR8 cloning vector using the TOPO™ TA Cloning Kit (Thermo Fisher, K250020) for stabilization and sequencing. Putative nos2 promoter region was excised from the TOPO vector using EcoRI and SmaI, treated with T4 polymerase, then inserted into SmaI-linearized p5E-MCS (Addgene, #26029).
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Source: Addgene
