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URL: http://www.addgene.org/23013
Proper Citation: RRID:Addgene_23013
Insert Name: I-CreI homing endonuclease
Organism: Chlamydomonas reinhardtii
Bacterial Resistance: Tetracycline
Defining Citation: PMID:19165721
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8900; Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: Seligman, LM et al., 1997, Genetics, 147: 1653-1664 The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations. Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function.
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Source: Addgene
