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URL: http://www.addgene.org/232482
Proper Citation: RRID:Addgene_232482
Insert Name: Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
Organism: Streptococcus pyogenes
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:40293262
Vector Backbone Description: Backbone Size:9357; Vector Backbone:pJAK184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Comments: Plasmid contains up- and down-stream homologous regions to insert dCas9 under TetR control into the genome downstream of the bacitracin permease. The promoter driving dCas9 has been modified to provide sufficient induction on a single copy system without appreciable 'leakiness' when not induced.
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No rating or validation information has been found for pJAK184.tetR.PT5-3.dCas9.
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Source: Addgene
