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URL: http://www.addgene.org/232752
Proper Citation: RRID:Addgene_232752
Insert Name: FlpO
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:42062565
Vector Backbone Description: Backbone Size:1725; Vector Backbone:pUC19; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Comments: PCR template for generating linear template for in vitro transcription. 5' and 3' beta globin UTR sequences were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290 The primers used for generating PCR template also came from this work, and are as follows: FWD: 5’- AGCTATAATACGACTCACTATAAGctcctgggcaacgtgctg-3’, which encodes the T7 promoter compatible with AG CleanCap reagent (upper-case bases) and binds to the 5’ UTR (lower-case bases) REV: 5’-poly(T)116-GCAATGAAAATAAATGTTTTTTATTAGGCAGAAT-3’}, which encodes the poly(A) tail and binds to the 3’-UTR.
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Source: Addgene
