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URL: http://www.addgene.org/234714
Proper Citation: RRID:Addgene_234714
Bacterial Resistance: Chloramphenicol and Ampicillin
Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
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Source: Addgene
