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URL: http://www.addgene.org/235443
Proper Citation: RRID:Addgene_235443
Insert Name: sfGFP Dropout Cassette
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
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Source: Addgene
