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Plasmid Name
PHO4NLS-GFP-PRA (109)
RRID:Addgene_24044 RRID Copied  
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RRID:Addgene_24044
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Plasmid Information

URL: http://www.addgene.org/24044

Proper Citation: RRID:Addgene_24044

Insert Name: PHO4

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Size:9000; Vector Backbone:pYX242; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin

Comments: NLS of PHO4 (bp 421-588) inserted in EcoRI/BamHI, eGFP inserted downstream in BamHI/HindIII and a single PrA repeat inserted further downstream in HindIII/SalI of pYX242. PrA motif sequence downstream of GFP: GCTCAACAAAATGCTTTTTATCAAGTCTTAAATATGCCTAACTTAAATGCTGATCAACGCAATGGTTTTATCCAAAGCCTTAAAGATGATCCAAGCCAAAGTGCTAACGTTTTAGGTGAAGCTCAAAAACTTAATGACTCTCAAGCTCCAAAAGCTGATGCGCAACAAAATAAC

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Data and Source Information

Source: Addgene