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URL: http://www.addgene.org/240644
Proper Citation: RRID:Addgene_240644
Insert Name: AIPGENE865 promoter
Organism: Exaiptasia diaphana
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Tim Howes; Backbone Size:3366; Vector Backbone:pT2HE; Vector Types:Gene expression and genomic integration in fish; Bacterial Resistance:Ampicillin
Comments: The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid. AmNeonGreen is a version of mNeonGreen codon-optimized for Aiptasia (based on the transcriptome of Lehnert et al., 2014) with the identical protein sequence. Lehnert EM, Mouchka ME, Burriesci MS, Gallo ND, Schwarz JA, Pringle JR. 2014. Extensive differences in gene expression between symbiotic and aposymbiotic cnidarians. G3 Bethesda Md. 4(2):277–295. doi:10.1534/g3.113.009084.
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Source: Addgene
