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URL: http://www.addgene.org/24336
Proper Citation: RRID:Addgene_24336
Insert Name: 5xQUAS/GFP split with an intron (GG)
Organism: Neurospora crassa/other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20434990
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp long, shown in capital letters, with spacer sequences in small letters): GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG CTTATCCtcGGGTAATCGCTTATCCtt This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP split with an intron (GG) (Zong et al., 2005)
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Source: Addgene
