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URL: http://www.addgene.org/24536
Proper Citation: RRID:Addgene_24536
Insert Name: ECFP-IETD2x-Venus
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:19053173
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Initiator caspase reporter protein (IC-RP) carries tandem copies of IETD in its linker, a sequence that is efficiently cleaved by caspase-8, but poorly by caspases-3 and -7. IETD constitutes the site in procaspase-3 for initiator caspase cleavage, and IC-RP cleavage is therefore a good readout of procaspase-3 activation. IC-RP was constructed by ligating Venus(YFP) between BamHI and EcoRI sites of pECFP-C1 and ligating linkers encoding cleavage sequences as BspEI-BamHI fragments between ECFP and Venus. Multiple serine and glycine residues flanking the cleavage sequences were added to increase linker flexibility and substrate accessibility. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information
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Source: Addgene
