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Plasmid Name
pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis
RRID:Addgene_246982 RRID Copied  
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RRID:Addgene_246982
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Plasmid Information

URL: http://www.addgene.org/246982

Proper Citation: RRID:Addgene_246982

Insert Name: φC31(PhiC31)

Organism: Other

Bacterial Resistance: Kanamycin

Vector Backbone Description: Backbone Marker:NA; Backbone Size:5218; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin

Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease). The total insert size displayed on this page (2403bp) includes all tags and linkers.

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Ratings and Alerts

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Data and Source Information

Source: Addgene