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URL: http://www.addgene.org/248335
Proper Citation: RRID:Addgene_248335
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Intermediate vector, referred to as a gRNA insert vector, is used to clone guide RNA sequences by annealing complementary oligonucleotides with appropriate overhangs and inserting them into a BbsI site positioned between the PpU6 promoter and the gRNA scaffold. The PpU6 promoter and gRNA scaffold are flanked by outward-facing BsaI sites with unique four-base overhangs, enabling directional and ordered assembly via Golden Gate.
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No rating or validation information has been found for EKv406(Gold Cas-guide insert2).
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Source: Addgene
