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URL: http://www.addgene.org/24882
Proper Citation: RRID:Addgene_24882
Insert Name: Pirh2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19043414
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX-2TKm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli strain for expression: BL21DE3 Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers. Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours. For pGEX-2TKm vector, the kinase site RRASV is removed and NdeI site is inserted at 5' of BamHI.
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No rating or validation information has been found for human pirh2-(138-261).
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Source: Addgene
