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URL: http://www.addgene.org/253620
Proper Citation: RRID:Addgene_253620
Insert Name: FLP recombinase
Bacterial Resistance: Spectinomycin
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: Alternative name: pRC453 Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain. Once pSC01 is integrated into the chromosome, this plasmid expresses FLP recombinase to cure / excise the backbone marker and contains a SacB marker for sucrose counterselection. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.
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Source: Addgene
