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URL: http://www.addgene.org/255961
Proper Citation: RRID:Addgene_255961
Insert Name: FRT-SEC(pDD382)-mCherry-N' linker-SD-rFRT3
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pFH173; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: For unknown reasons, the DNA construct (pHW2159) seems toxic to the E. coli strain DH5α, often resulting in unexpected mutations. When transforming this plasmid into DH5α, we recommend selecting only small colonies and sequencing the plasmid following fresh miniprep from a bacterial culture to confirm absence of any mutations. To overcome this issue, the deposited bacterial stock of this plasmid is NEB® Turbo competent E. coli, which better tolerates this construct and more reliably yield plasmid DNA with the correct sequence.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
