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URL: http://www.addgene.org/25742
Proper Citation: RRID:Addgene_25742
Insert Name: G alpha 13 and 12 miR-shRNA
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16945906
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12752; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: pSLIK-Venus with G alpha 12 and 13 miR-shRNA G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT G alpha 13 miR-shRNA: CTGGGTGAGTCTGTAAAGTATT There are 3 minor changes between depositor's sequence and Addgene's sequence - An extra T between the TRE promoter and first shRNA; a 17nt stretch between the shRNAs is different but will not affect function; and a single nt change mutates an EcoRI site just beyond the 2nd shRNA but will again not affect function.
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Source: Addgene
