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URL: http://www.addgene.org/25800
Proper Citation: RRID:Addgene_25800
Insert Name: alternative hsa-miR-34a promoter
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19584398
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: promoter region of the alternative pri-miR-34a transcript identified by Navarro et al. Blood 2009 Sep 3;114(10):2181-92. There are several discrepancies between this sequence and the GenBank sequence: T(at position 388) is cahnged to C "ACACAC" insertion at position 658 "T" insertion at position 1914 A (at position 1599) changed to G T (at position 1480) changed to G G (at position 1212) changed to A T (at position 1199) changed to C These changes were present in all the clones that the lab sequenced and these clones came from two independent PCRs using genomic DNA extracted from the human erithroleukemia cell line K562. The changes might be due to that particular cell line. Importantly, all the minipreps I tested induced luciferase activity in K562 cells upon TPA treatment (as reported in the manuscript).
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Source: Addgene
