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Plasmid Name
Mtb-Timer
RRID:Addgene_258482 RRID Copied  
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RRID:Addgene_258482
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Plasmid Information

URL: http://www.addgene.org/258482

Proper Citation: RRID:Addgene_258482

Insert Name: TIMER

Organism: Synthetic

Bacterial Resistance: Hygromycin

Defining Citation: PMID:42153669

Vector Backbone Description: Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin

Comments: Timer protein, a derivative of DsRed, undergoes a spontaneous and irreversible green-to-red emission shift as it matures over time. Newly synthesized Timer molecules emit green fluorescence, which gradually shifts to red over time. Bacteria can be identified as actively replicating (green), intermediate (orange), or non-replicating (red) based on their green-to-red fluorescence ratio.

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Data and Source Information

Source: Addgene