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URL: http://www.addgene.org/258581
Proper Citation: RRID:Addgene_258581
Insert Name: PtGFP
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16600023
Vector Backbone Description: Backbone Marker:Andrew Gleave (Addgene #247985); Backbone Size:5042; Vector Backbone:pART7; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Ampicillin
Comments: pART7::COX:PtGFP is for targeting PtGFP to the plant mitochondria matrix. The PtGFP gene is concatenated downstream of a sequence coding for the signal sequence (first 29 aminoacids) of the cytochrome oxidase subunit IV from yeast (COX-IV; Gene Bank X01418; Maarse et al. 1984, doi.org/10.1002/j.1460-2075.1984.tb02216.x; Akashi et al. 1998, doi.org/10.1016/S0014-5793(98)00717-0; Filipin et al. 2005, doi.org/10.1016/j.ceca.2004.08.002; Llopis et al. 1998, doi.org/10.1073/pnas.95.12.6803 ). A 5'UTR 144 nucleotide translation leader sequence from the tobacco etch virus (TEV; Gene Bank GU734657) was prepended for enhancement of expression (Carrington and Freed 1990 doi.org/10.1128/jvi.64.4.1590-1597.1990; Zeenko and Gallie 2005, DOI: 10.1074/jbc.M503576200; Peeters et al. 2000, doi.org/10.1007/s002390010044; Harper et al. 1998, doi.org/10.1074/jbc.273.2.1099). PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
