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URL: http://www.addgene.org/26301
Proper Citation: RRID:Addgene_26301
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Vector Backbone Description: Backbone Size:5879; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Use for puromycin selection of transgenes in C. elegans and related species if transgene has a visual phenotype and Pmyo-2::mCherry expression is undesirable. The unc-119 gene in pCG150 was replaced by the Prpl-28::PuroR::let-858_3'UTR cassette for puromycin selection in worms. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
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Source: Addgene
