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URL: http://www.addgene.org/27126
Proper Citation: RRID:Addgene_27126
Insert Name: Colicin Ia + Ia immunity protein
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11004207
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the C-terminal channel forming domain of colicin Ia and the Ia immunity protein. The construct is also referred to as "CT-Medium or CT-M". The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
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Source: Addgene
