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URL: http://www.addgene.org/27408
Proper Citation: RRID:Addgene_27408
Insert Name: SPL10 mutant
Organism: Arabidopsis thaliana
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21123653
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Comments: Created by cloning 4.9-kb (including 1.8 kb upstream of CDS, CDS, and 1.6 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick- Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-CCATGAATCTCATGATTTCTACAGTGCACTGTCATTGCTCTCAACTACTTCGGATTCACAAGGGATC-3' and 5'-GATCCCTTGTGAATCCGAAGTAGTTGAGAGCAATGACAGTGCACTGTAGAAATCATGAGATTCATGG-3'). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
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No rating or validation information has been found for 6mpSPL10F13R13::pBIB-BAS (6mSPL10).
No alerts have been found for 6mpSPL10F13R13::pBIB-BAS (6mSPL10).
Source: Addgene
