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URL: http://www.addgene.org/27409
Proper Citation: RRID:Addgene_27409
Insert Name: SPL10 inverted
Organism: Arabidopsis thaliana
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21123653
Vector Backbone Description: Backbone Size:0; Vector Backbone:UBI3pTpFSPGW-HYGv02; Vector Types:; Bacterial Resistance:Kanamycin
Comments: SPL10-RNAi constructs were generated by amplifying 0.6kb of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article.
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No rating or validation information has been found for UB13p::dsSPL10 v01 (SPL10-RNAi).
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Source: Addgene
