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URL: http://www.addgene.org/27791
Proper Citation: RRID:Addgene_27791
Insert Name: Amber-Cerulean-Venus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:19946626
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Stoichiometry standard; Bacterial Resistance:Kanamycin
Comments: Amber can be excised using a NheI-BSP E1 double digest; Cerulean by BglII-SalI and Venus by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest. Due to the multiple fluorescent protein repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
