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Plasmid Name
RSRF-Luc-2mut
RRID:Addgene_31819 RRID Copied  
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RRID:Addgene_31819
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Plasmid Information

URL: http://www.addgene.org/31819

Proper Citation: RRID:Addgene_31819

Insert Name: Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:7565789

Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin

Comments: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The mutated RSRF sequence (two CTATATTTAG RSRF sites mutated to CGATATTTCG) of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.

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Data and Source Information

Source: Addgene