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URL: http://www.addgene.org/32600
Proper Citation: RRID:Addgene_32600
Insert Name: mRFP1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15996270
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The mRFP1 coding sequence was amplified by PCR using primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and 3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx Polymerase (Invitrogen). The resulting product was cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene 6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and verify that it produced robust red fluorescence More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12)
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Source: Addgene
