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Plasmid Name
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
RRID:Addgene_35191 RRID Copied  
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RRID:Addgene_35191
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Plasmid Information

URL: http://www.addgene.org/35191

Proper Citation: RRID:Addgene_35191

Insert Name: loxP KAN loxP 24xPP7SL MDN1

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Ampicillin

Defining Citation: PMID:21512033

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin

Comments: This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid. Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert. Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.

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Ratings and Alerts

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Data and Source Information

Source: Addgene