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URL: http://www.addgene.org/35194
Proper Citation: RRID:Addgene_35194
Insert Name: PCP-2x-yeGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21512033
Vector Backbone Description: Vector Backbone:pURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid expresses a chimeric RNA-binding protein fused to GFP used to detect mRNAs that contain multiple copies of an RNA sequence (see Addgene plasmid #'s 35191, 35192 & 35196). See associated publication for detailed methods and use of this plasmid. Please note that Addgene's sequencing results identified discrepancies when compared to the assembled sequence provided by the depositing laboratory. The first GFP construct in this plasmid (bp#441-1154) is yeast codon optimized and contains a E124K mutation and amino acid 206 is A (not K, as suggested by the depositor's sequence). The second GFP construct in this plasmid (bp# 1212-1928) is NOT yeast codon optimized. This construct has successfully been used by the depositing laboratory as detailed in the associated publication and the sequencing discrepancies are not a concern for the function of the plasmid.
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No rating or validation information has been found for pDZ276 (pURA MET28 PCP-2x-yeGFP).
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Source: Addgene
